A role for beta-actin in RNA polymerase III transcription

Hu, P., Wu, S., Hernandez, N. (December 2004) A role for beta-actin in RNA polymerase III transcription. Genes Dev, 18 (24). pp. 3010-5. ISSN 0890-9369 (Print)

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URL: http://genesdev.cshlp.org/content/18/24/3010
DOI: 10.1101/gad.1250804


When transcription from the human U6 snRNA gene is reconstituted with recombinant factors and purified RNA polymerase III (pol III), pol III must be treated with CK2 to be active. We show that highly purified pol III contains associated beta-actin, and beta-actin localizes to an active U6 promoter in vivo. Pol III immunoprecipitated from IMR90 cells treated with a genotoxic agent lacks associated beta-actin and is inactive in the reconstituted assay. Transcription is regained upon treatment of pol III with CK2 and addition of beta-actin. This suggests that beta-actin associated with pol III is essential for basal pol III transcription.

Item Type: Paper
Uncontrolled Keywords: Actins metabolism Casein Kinase II metabolism Cells Cultured Humans Immunoprecipitation Microscopy Fluorescence Promoter Regions (Genetics) genetics RNA Polymerase III/genetics metabolism RNA, Small Nuclear genetics Transcription Genetic genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
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Depositing User: CSHL Librarian
Date: 15 December 2004
Date Deposited: 03 Feb 2012 15:17
Last Modified: 03 Feb 2012 15:17
URI: https://repository.cshl.edu/id/eprint/22394

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