RNA recognition motif 2 directs the recruitment of SF2/ASF to nuclear stress bodies

Chiodi, I., Corioni, M., Giordano, M., Valgardsdottir, R., Ghigna, C., Cobianchi, F., Xu, R. M., Riva, S., Biamonti, G. (August 2004) RNA recognition motif 2 directs the recruitment of SF2/ASF to nuclear stress bodies. Nucleic Acids Research, 32 (14). pp. 4127-4136. ISSN 0305-1048

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Heat shock induces the transcriptional activation of large heterochromatic regions of the human genome composed of arrays of satellite III DNA repeats. A number of RNA-processing factors, among them splicing factor SF2/ASF, associate with these transcription factors giving rise to nuclear stress bodies (nSBs). Here, we show that the recruitment of SF2/ASF to these structures is mediated by its second RNA recognition motif. Amino acid substitutions in the first a-helix of this domain, but not in the P-strand regions, abrogate the association with nSBs. The same mutations drastically affect the in vivo activity of SF2/ASF in the alternative splicing of adenoviral E1A transcripts. Sequence analysis identifies four putative high-affinity binding sites for SF2/ASF in the transcribed strand of the satellite III DNA. We have verified by gel mobility shift assays that the second RNA-binding domain of SF2/ASF binds at least one of these sites. Our analysis suggests that the recruitment of SF2/ASF to nSBs is mediated by a direct interaction with satellite III transcripts and points to the second RNA-binding domain of the protein as the major determinant of this interaction.

Item Type: Paper
Uncontrolled Keywords: PRE-MESSENGER-RNA pre-messenger RNA ALTERNATIVE SPLICING SPECIFICITY alternative splicing specificity HEAT-SHOCK heat shock IN-VIVO in-vivo SR PROTEINS SR proteins TRANSCRIPTION FACTORS transcription factors MODULAR DOMAINS modular domains BINDING binding DOMAIN FACTOR ASF/SF2 factor SITES
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
CSHL Authors:
Depositing User: CSHL Librarian
Date: August 2004
Date Deposited: 09 Feb 2012 15:58
Last Modified: 09 Feb 2012 15:58
URI: https://repository.cshl.edu/id/eprint/22348

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