MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles

Laguillo-Diego, Alejandra, Kiewisz, Robert, Martí-Gómez, Carlos, Baum, Daniel, Müller-Reichert, Thomas, Vernos, Isabelle (November 2022) MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles. Molecular Biology of the Cell. mbcE22080306T. ISSN 1059-1524

Abstract

Faithful chromosome segregation requires the assembly of a bipolar spindle, consisting of two antiparallel microtubule (MT) arrays having most of their minus ends focused at the spindle poles and their plus ends overlapping in the spindle midzone. Spindle assembly, chromosome alignment and segregation require highly dynamic MTs. The plus ends of MTs have been extensively investigated; instead, their minus end structure remains poorly characterized. Here, we used large-scale electron tomography to study the morphology of the MT minus ends in 3D-reconstructed metaphase spindles in HeLa cells. In contrast to the homogeneous open morphology of the MT plus ends at the kinetochores, we found that MT minus ends are heterogeneous showing either open or closed morphologies. Silencing the minus-end specific stabilizer, MCRS1 increased the proportion of open MT minus ends. Altogether, these data suggest a correlation between the morphology and the dynamic state of the MT ends. Taking this heterogeneity of the MT minus end morphologies into account, our work indicates an unsynchronized behavior of MTs at the spindle poles, thus laying the ground for further studies on the complexity of MT dynamics regulation. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text].

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > microtubule organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > mitotic spindle organs, tissues, organelles, cell types and functions organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions
CSHL Authors:	Marti Gomez Aldaravi Carlos
Communities:	CSHL labs > McCandlish lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	9 November 2022
Date Deposited:	02 Dec 2022 15:43
Last Modified:	11 Jan 2024 20:58
PMCID:	PMC9816640
URI:	https://repository.cshl.edu/id/eprint/40759

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