Homologous organization of cerebellar pathways to sensory, motor, and associative forebrain.

Pisano, Thomas J, Dhanerawala, Zahra M, Kislin, Mikhail, Bakshinskaya, Dariya, Engel, Esteban A, Hansen, Ethan J, Hoag, Austin T, Lee, Junuk, de Oude, Nina L, Venkataraju, Kannan Umadevi, Verpeut, Jessica L, Hoebeek, Freek E, Richardson, Ben D, Boele, Henk-Jan, Wang, Samuel S-H (September 2021) Homologous organization of cerebellar pathways to sensory, motor, and associative forebrain. Cell reports, 36 (12). p. 109721. ISSN 2211-1247

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Abstract

Cerebellar outputs take polysynaptic routes to reach the rest of the brain, impeding conventional tracing. Here, we quantify pathways between the cerebellum and forebrain by using transsynaptic tracing viruses and a whole-brain analysis pipeline. With retrograde tracing, we find that most descending paths originate from the somatomotor cortex. Anterograde tracing of ascending paths encompasses most thalamic nuclei, especially ventral posteromedial, lateral posterior, mediodorsal, and reticular nuclei. In the neocortex, sensorimotor regions contain the most labeled neurons, but we find higher densities in associative areas, including orbital, anterior cingulate, prelimbic, and infralimbic cortex. Patterns of ascending expression correlate with c-Fos expression after optogenetic inhibition of Purkinje cells. Our results reveal homologous networks linking single areas of the cerebellar cortex to diverse forebrain targets. We conclude that shared areas of the cerebellum are positioned to provide sensory-motor information to regions implicated in both movement and nonmotor function.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > animal organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex organs, tissues, organelles, cell types and functions > tissues types and functions > cerebellum organs, tissues, organelles, cell types and functions > tissues types and functions > forebrain bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types organism description > animal > mammal organism description > animal > mammal > rodent > mouse neurobiology neurobiology > neuroscience bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogenes organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent neurobiology > neuroscience > systems neuroscience organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Umadevi Venkataraju, Kannan
Communities:	CSHL labs > Osten lab
Depositing User:	Sasha Luks-Morgan
Date:	21 September 2021
Date Deposited:	30 Sep 2021 18:14
Last Modified:	25 Jan 2024 16:15
PMCID:	PMC8506234
URI:	https://repository.cshl.edu/id/eprint/40376

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