An Optimized Whole-Mount Immunofluorescence Method for Shoot Apices.

Tran, Thu M, Demesa-Arevalo, Edgar, Kitagawa, Munenori, Garcia-Aguilar, Marcelina, Grimanelli, Daniel, Jackson, David (April 2021) An Optimized Whole-Mount Immunofluorescence Method for Shoot Apices. Curr Protoc, 1 (4). e101. ISSN 2691-1299

DOI: 10.1002/cpz1.101


The localization of a protein provides important information about its biological functions. The visualization of proteins by immunofluorescence has become an essential approach in cell biology. Here, we describe an easy-to-follow immunofluorescence protocol to localize proteins in whole-mount tissues of maize (Zea mays) and Arabidopsis. We present the whole-mount immunofluorescence procedure using maize ear primordia and Arabidopsis inflorescence apices as examples, followed by tips and suggestions for each step. In addition, we provide a supporting protocol to describe the use of an ImageJ plug-in to analyze colocalization. This protocol has been optimized to observe proteins in 2-5 mm maize ear primordia or in developing Arabidopsis inflorescence apices; however, it can be used as a reference to perform whole-mount immunofluorescence in other plant tissues and species. © 2021 Wiley Periodicals LLC. Basic Protocol: Whole-mount immunofluorescence for maize and Arabidopsis shoot apices Support Protocol: Measure colocalization by JACoP plugin in ImageJ.

Item Type: Paper
Subjects: organism description > plant > Arabidopsis
organism description > plant > maize
Investigative techniques and equipment > microscopy > immunoflourescence microscopy
CSHL Authors:
Communities: CSHL labs > Jackson lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: April 2021
Date Deposited: 30 Apr 2021 19:54
Last Modified: 30 Apr 2021 19:54

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