Xu, X., Crow, M., Rice, B. R., Li, F., Harris, B., Liu, L., Demesa-Arevalo, E., Lu, Z., Wang, L., Fox, N., Wang, X., Drenkow, J., Luo, A., Char, S. N., Yang, B., Sylvester, A. W., Gingeras, T. R., Schmitz, R. J., Ware, D., Lipka, A. E., Gillis, J., Jackson, D. (December 2020) Single-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery. Dev Cell, S1534- (20). pp. 31021-31022. ISSN 1534-5807
Abstract
Crop productivity depends on activity of meristems that produce optimized plant architectures, including that of the maize ear. A comprehensive understanding of development requires insight into the full diversity of cell types and developmental domains and the gene networks required to specify them. Until now, these were identified primarily by morphology and insights from classical genetics, which are limited by genetic redundancy and pleiotropy. Here, we investigated the transcriptional profiles of 12,525 single cells from developing maize ears. The resulting developmental atlas provides a single-cell RNA sequencing (scRNA-seq) map of an inflorescence. We validated our results by mRNA in situ hybridization and by fluorescence-activated cell sorting (FACS) RNA-seq, and we show how these data may facilitate genetic studies by predicting genetic redundancy, integrating transcriptional networks, and identifying candidate genes associated with crop yield traits.
| Item Type: | Paper |
|---|---|
| Additional Information: | 1878-1551 Xu, Xiaosa Crow, Megan Rice, Brian R Li, Forrest Harris, Benjamin Liu, Lei Demesa-Arevalo, Edgar Lu, Zefu Wang, Liya Fox, Nathan Wang, Xiaofei Drenkow, Jorg Luo, Anding Char, Si Nian Yang, Bing Sylvester, Anne W Gingeras, Thomas R Schmitz, Robert J Ware, Doreen Lipka, Alexander E Gillis, Jesse Jackson, David Journal Article United States Dev Cell. 2020 Dec 30:S1534-5807(20)31021-2. doi: 10.1016/j.devcel.2020.12.015. |
| Subjects: | organism description > plant > maize Investigative techniques and equipment > assays > Single cell sequencing |
| CSHL Authors: | |
| Communities: | CSHL labs > Gillis Lab CSHL labs > Gingeras lab CSHL labs > Jackson lab CSHL labs > Ware lab School of Biological Sciences > Publications |
| Depositing User: | Matthew Dunn |
| Date: | 30 December 2020 |
| Date Deposited: | 11 Jan 2021 14:35 |
| Last Modified: | 01 Jun 2022 17:27 |
| PMCID: | PMC7904613 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/39889 |
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