The retinoblastoma protein is phosphorylated during specific phases of the cell cycle

Buchkovich, K., Duffy, L. A., Harlow, E. (September 1989) The retinoblastoma protein is phosphorylated during specific phases of the cell cycle. Cell, 58 (6). pp. 1097-105. ISSN 1097-4172

DOI: 10.1016/0092-8674(89)90508-4


p105-RB is the product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to act as an inhibitor of cellular proliferation, yet surprisingly it is present in actively dividing cells. To look for changes in p105-RB that may regulate its activity during the cell cycle, we generated synchronized cell populations and followed their progression through the cell cycle. p105-RB is synthesized throughout the cycle, but is phosphorylated in a phase-specific manner. In the G0 and G1 phases of the cell cycle, an unphosphorylated species of the protein is the only detectable form, whereas in the S and G2/M phases, multiple phosphorylated species of p105-RB are detected.

Item Type: Paper
Uncontrolled Keywords: *Cell Cycle DNA Replication Eye Neoplasms/*genetics Flow Cytometry Hela Cells/cytology/metabolism Humans Immunoblotting Interphase Mitosis Phosphoproteins/biosynthesis/*metabolism Phosphorylation Research Support, U.S. Gov't, P.H.S. Retinoblastoma/*genetics Retinoblastoma Protein *Suppression, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
diseases & disorders > cancer > cancer types > retinoblastoma
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: 22 September 1989
Date Deposited: 28 Jul 2017 17:22
Last Modified: 28 Jul 2017 17:22
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