CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals

Mariani, R., Skowronski, J. (June 1993) CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals. Proc Natl Acad Sci U S A, 90 (12). pp. 5549-53. ISSN 0027-8424 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/8516299

Abstract

PCR was used to clone isolates of the human immunodeficiency virus type 1 (HIV-1) nef gene directly from peripheral blood leukocytes of HIV-1-infected individuals. A transient expression system with human CEM T cells was used to assess the effect of nef on CD4 antigen expression on the cell surface. We show that CD4 down-regulation is a frequent property of primary HIV-1 nef alleles. Mutations in conserved amino acid motifs of Nef disrupted CD4 down-regulation. Our observations strongly suggest that CD4 down-regulation reflects a conserved function of nef, which is selected in vivo in human HIV-1 infection. Methodology described here provides quantitative assays to establish whether alterations in nef correlate with the dynamics of disease progression in human AIDS.

Item Type: Paper
Uncontrolled Keywords: Acquired Immunodeficiency Syndrome/ immunology Alleles Amino Acid Sequence Antigens, CD4/ metabolism Base Sequence DNA, Viral/blood/genetics/isolation & purification Down-Regulation Gene Products, nef/biosynthesis/genetics Genes, nef HIV-1/ genetics/ isolation & purification Humans Leukocytes/physiology Molecular Sequence Data Mutation Oligodeoxyribonucleotides Open Reading Frames Polymerase Chain Reaction Research Support, Non-U.S. Gov't
Subjects: diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
CSHL Authors:
Communities: CSHL labs > Skowronski lab
Depositing User: Matt Covey
Date: 15 June 1993
Date Deposited: 08 Apr 2016 19:54
Last Modified: 09 Nov 2017 20:00
PMCID: PMC46758
Related URLs:
URI: https://repository.cshl.edu/id/eprint/32590

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