Stern, S., Herr, W. (December 1991) The herpes simplex virus trans-activator VP16 recognizes the Oct-1 homeo domain: evidence for a homeo domain recognition subdomain. Genes Dev, 5 (12B). pp. 2555-66. ISSN 0890-9369 (Print)0890-9369 (Linking)
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Abstract
The homeo domain of the Oct-1 transcription factor directs formation of a multiprotein-DNA complex containing Oct-1, the herpes simplex virus (HSV) trans-activator VP16, and a second host cell factor (HCF). This VP16-induced complex alters the regulatory activity of Oct-1, in part, by associating it with the potent VP16 acidic transcriptional activation domain. Here, we show that in the absence of HCF, VP16 can recognize specifically the Oct-1 homeo domain. A region of VP16 near the acidic activation domain appears to be involved exclusively in homeo domain recognition because a 4-amino-acid insertion within this region only affects the ability of VP16 to interact with Oct-1, leaving its DNA- and HCF-binding activities unchanged. A 33-amino-acid peptide containing this region complexes with the Oct-1 POU domain bound to DNA, suggesting that this VP16 region contains an autonomous homeo domain recognition subdomain.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence Carrier Proteins/genetics DNA, Viral/chemistry DNA-Binding Proteins/*genetics *Genes, Homeobox Herpes Simplex Virus Protein Vmw65 Host Cell Factor C1 Integration Host Factors Molecular Sequence Data Mutation Octamer Transcription Factor-1 Simplexvirus/*genetics Trans-Activators/*genetics Transcription Factors/*genetics Transcription, Genetic Viral Proteins/*genetics |
| Subjects: | organism description > virus > herpes simplex virus bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > homeodomain protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Herr lab |
| Depositing User: | Matt Covey |
| Date: | December 1991 |
| Date Deposited: | 07 Jan 2016 17:11 |
| Last Modified: | 03 Nov 2017 20:50 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32084 |
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