Investigation of acquired resistance to EGFR-targeted therapies in lung cancer using cDNA microarrays

Kani, K., Sordella, R., Mallick, P. (2012) Investigation of acquired resistance to EGFR-targeted therapies in lung cancer using cDNA microarrays. Methods in Molecular Biology, 795. pp. 233-53. ISSN 1940-6029 (Electronic)1064-3745 (Linking)

Abstract

Clinical tools to accurately describe, evaluate, and predict an individual's response to cancer therapy are a field-wide priority; in many advanced cancers, only 10-20% of individuals will have a clinical benefit from therapy, yet we treat the entire population. Furthermore, many therapies are initially effective, but lose effectiveness over time. Here we describe methods to derive in vitro models of resistance to EGFR tyrosine kinase inhibitors. We additionally describe approaches to characterize possible mechanisms of resistance by genomic and transcriptomic approaches.

Item Type:	Paper
Uncontrolled Keywords:	Antineoplastic Agents/ pharmacology/therapeutic use Carcinoma, Non-Small-Cell Lung/drug therapy/ genetics Cell Line, Tumor Drug Resistance, Neoplasm/drug effects/genetics Epithelial-Mesenchymal Transition/genetics Gene Expression Profiling Genetic Markers/genetics Humans Lung Neoplasms/drug therapy/ genetics Oligonucleotide Array Sequence Analysis Protein Kinase Inhibitors/ pharmacology/therapeutic use Quinazolines/pharmacology Receptor, Epidermal Growth Factor/antagonists & inhibitors/ genetics Sequence Analysis, DNA
Subjects:	bioinformatics > genomics and proteomics > analysis and processing diseases & disorders > cancer diseases & disorders bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification therapies therapies > cancer drugs - see diseases-cancer-drugs and therapies bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > epidermal growth factor diseases & disorders > cancer > cancer types > lung cancer bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types diseases & disorders > cancer > cancer types
CSHL Authors:	Sordella, Raffaella
Communities:	CSHL labs > Sordella lab CSHL Cancer Center Program > Signal Transduction
Depositing User:	Matt Covey
Date:	2012
Date Deposited:	13 Mar 2013 14:40
Last Modified:	16 Oct 2015 14:07
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27785

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