Caspases disrupt the nuclear-cytoplasmic barrier

Faleiro, L., Lazebnik, Y. (2000) Caspases disrupt the nuclear-cytoplasmic barrier. J Cell Biol, 151 (5). pp. 951-959. ISSN 0021-9525

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DOI: 10.1083/jcb.151.5.951


During apoptosis, caspases, a family of proteases, disassemble a cell by cleaving a set of proteins. Caspase-3 plays a major role in the dissassembly of the nucleus by processing several nuclear substrates. The question is how caspase-3 which is usually cytoplasmic, gains access to its nuclear targets. It was suggested that caspase-3 is actively transported to the nucleus through the nuclear pores. We found that caspase-9, which is activated earlier than caspase-3, directly or indirectly inactivates nuclear transport and increases the diffusion limit of the nuclear pores. This increase allows caspase-3 and other molecules that could not pass through the nuclear pores in living cells to enter or leave the nucleus during apoptosis by diffusion. Hence, caspase-9 contributes to cell disassembly by disrupting the nuclear cytoplasmic barrier.

Item Type: Paper
Uncontrolled Keywords: Active Transport, Cell Nucleus Antineoplastic Agents Apoptosis Breast Neoplasms Caspase 3 Caspase 8 Caspase 9 Caspases Cell Compartmentation Cisplatin Cytoplasm Diffusion Female Humans Nerve Tissue Proteins Nuclear Pore Spectrin Tumor Cells, Cultured
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
CSHL Authors:
Communities: CSHL labs > Labeznik lab
Depositing User: Matt Covey
Date: 2000
Date Deposited: 12 Dec 2012 17:31
Last Modified: 12 Dec 2012 17:31
PMCID: PMC2174353
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