Direct Isolation of Neural Stem Cells in the Adult Hippocampus after Traumatic Brain Injury

Gao, X., Enikolopov, G. N., Chen, J. (2008) Direct Isolation of Neural Stem Cells in the Adult Hippocampus after Traumatic Brain Injury. J Neurotrauma, 25 (8). 985-995 .

URL: https://www.ncbi.nlm.nih.gov/pubmed/18665804
DOI: 10.1089/neu.2008.0460

Abstract

Recently, we have manipulated endogenous neural stem/progenitor cells (NSCs) in situ in the adult mouse to undergo neurogenesis and anatomic circuit re-formation de novo in the neocortex, where it does not normally occur, by using a highly targeted brain injury model. However, how the NSCs respond to injury in the adult mouse brain is poorly understood. While studying the molecular mechanisms that regulate NSC fates after brain injury, it is important to develop a strategy to identify NSCs in niches and isolate them directly from fresh tissue after brain injury. Here we report that we directly isolated NSCs from adult brains after traumatic brain injury by genetically labeling NSCs with EGFP combined with fluorescence-activated cell sorting (FACS) technique without an intervening cell culture and with high concentrations of growth factors. The isolated EGFP-positive cells can self-renew and have the potential to differentiate into both neurons and glia in vitro, confirming that the FACS-sorted EGFP-positive cells are NSCs. This unique approach provides a useful tool to isolate large amounts of endogenous NSCs in situ for identifying the critical molecules that regulate fate decision and neurogenesis in the adult brain after injury.

Item Type: Paper
Subjects: therapies > stem cells > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > germ cell > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > Adult

diseases & disorders > nervous system diseases and disorders
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therapies > stem cells
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: Tom Adams
Date Deposited: 11 Jul 2011 20:45
Last Modified: 28 Feb 2017 21:43
Related URLs:
URI: http://repository.cshl.edu/id/eprint/7741

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