Insulin receptor signaling regulates synapse number, dendritic plasticity, and circuit function in vivo

Chiu, S. L., Chen, C. M., Cline, H. T. (June 2008) Insulin receptor signaling regulates synapse number, dendritic plasticity, and circuit function in vivo. Neuron, 58 (5). pp. 708-719.

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URL: http://www.ncbi.nlm.nih.gov/pubmed/18549783
DOI: 10.1016/j.neuron.2008.04.014

Abstract

Insulin receptor signaling has been postulated to play a role in synaptic plasticity; however, the function of the insulin receptor in CNS is not clear. To test whether insulin receptor signaling affects visual system function, we recorded light-evoked responses in optic tectal neurons in living Xenopus tadpoles. Tectal neurons transfected with dominant-negative insulin receptor (dnIR), which reduces insulin receptor phosphorylation, or morpholino against insulin receptor, which reduces total insulin receptor protein level, have significantly smaller light-evoked responses than controls. dnIR-expressing neurons have reduced synapse density as assessed by EM, decreased AMPA mEPSC frequency, and altered experience-dependent dendritic arbor structural plasticity, although synaptic vesicle release probability, assessed by paired-pulse responses, synapse maturation, assessed by AMPA/NMDA ratio and ultrastructural criteria, are unaffected by dnIR expression. These data indicate that insulin receptor signaling regulates circuit function and plasticity by controlling synapse density.

Item Type: Paper
Uncontrolled Keywords: DEVBIO MOLNEURO SIGNALING PATHWAY
Subjects: organism description > animal > Frog
bioinformatics > genomics and proteomics > analysis and processing > NETBAG
?? Q1 ??
organism description > animal > developmental stage > child
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > tyrosine kinase
CSHL Authors:
Communities: CSHL labs > Cline lab
Watson School > Publications
Depositing User: Tom Adams
Date: 12 June 2008
Date Deposited: 13 Jul 2011 20:43
Last Modified: 22 Sep 2014 15:54
PMCID: PMC3057650
Related URLs:
URI: http://repository.cshl.edu/id/eprint/7709

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