Chang, K., Marran, K., Valentine, A., Hannon, G. J. (2012) RNAi in cultured mammalian cells using synthetic siRNAs. Cold Spring Harbor Protocols, 7 (9). pp. 957-961. ISSN 19403402
Abstract
RNA interference (RNAi) enables sequence-specific, experimentally induced silencing of almost any gene by tapping into innate regulatory mechanisms that are conserved among virtually all eukaryotes. In a typical RNAi experiment, an artificial silencing trigger directs the RNAi pathway toward a target that it would not normally recognize. This is most often an endogenous protein-coding gene, although some noncoding RNAs can also be silenced effectively. The artificial silencing trigger varies; this protocol uses synthetic small interfering RNAs (siRNAs). Lipofectamine 2000 is used to deliver the siRNAs into HEK293 cells. This lipid reagent has proven to be effective for many different cultured mammalian cell lines.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA silencing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing organism description > virus > viral transfection |
| CSHL Authors: | |
| Communities: | CSHL labs > Chang lab CSHL labs > Hannon lab |
| Depositing User: | Adrian Gomez |
| Date: | 2012 |
| Date Deposited: | 22 Nov 2019 20:35 |
| Last Modified: | 15 Nov 2023 16:57 |
| PMCID: | PMC3541682 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/38727 |
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