Double triage to identify poorly annotated genes in maize: The missing link in community curation

Tello-Ruiz, M. K., Marco, C. F., Hsu, F. M., Khangura, R. S., Qiao, P., Sapkota, S., Stitzer, M. C., Wasikowski, R., Wu, H., Zhan, J., Chougule, K., Barone, L. C., Ghiban, C., Muna, D., Olson, A. C., Wang, L., Ware, D., Micklos, D. A. (October 2019) Double triage to identify poorly annotated genes in maize: The missing link in community curation. PLoS ONE, 14 (10). Article number e0224086. ISSN 19326203 (ISSN)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=31658277
DOI: 10.1371/journal.pone.0224086

Abstract

The sophistication of gene prediction algorithms and the abundance of RNA-based evidence for the maize genome may suggest that manual curation of gene models is no longer necessary. However, quality metrics generated by the MAKER-P gene annotation pipeline identified 17,225 of 130,330 (13%) protein-coding transcripts in the B73 Reference Genome V4 gene set with models of low concordance to available biological evidence. Working with eight graduate students, we used the Apollo annotation editor to curate 86 transcript models flagged by quality metrics and a complimentary method using the Gramene gene tree visualizer. All of the triaged models had significant errors-including missing or extra exons, non-canonical splice sites, and incorrect UTRs. A correct transcript model existed for about 60% of genes (or transcripts) flagged by quality metrics; we attribute this to the convention of elevating the transcript with the longest coding sequence (CDS) to the canonical, or first, position. The remaining 40% of flagged genes resulted in novel annotations and represent a manual curation space of about 10% of the maize genome (~4,000 protein-coding genes). MAKER-P metrics have a specificity of 100%, and a sensitivity of 85%; the gene tree visualizer has a specificity of 100%. Together with the Apollo graphical editor, our double triage provides an infrastructure to support the community curation of eukaryotic genomes by scientists, students, and potentially even citizen scientists. © 2019 This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

Item Type: Paper
Subjects: organism description > plant > maize
bioinformatics > computational biology > algorithms
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
CSHL Authors:
Communities: CSHL labs > Ware lab
Dolan DNA Learning Center
Depositing User: Adrian Gomez
Date: 28 October 2019
Date Deposited: 20 Nov 2019 19:15
Last Modified: 20 Nov 2019 19:19
PMCID: PMC6816542
Related URLs:
URI: http://repository.cshl.edu/id/eprint/38716

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