Sorghum MSD3 Encodes an omega-3 Fatty Acid Desaturase that Increases Grain Number by Reducing Jasmonic Acid Levels

Dampanaboina, L., Jiao, Y., Chen, J., Gladman, N., Chopra, R., Burow, G., Hayes, C., Christensen, S. A., Burke, J., Ware, D., Xin, Z. (October 2019) Sorghum MSD3 Encodes an omega-3 Fatty Acid Desaturase that Increases Grain Number by Reducing Jasmonic Acid Levels. Int J Mol Sci, 20 (21). ISSN 1422-0067

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URL: https://www.ncbi.nlm.nih.gov/pubmed/31661847
DOI: 10.3390/ijms20215359

Abstract

Grain number per panicle is an important component of grain yield in sorghum (Sorghum bicolor (L.)) and other cereal crops. Previously, we reported that mutations in multi-seeded 1 (MSD1) and MSD2 genes result in a two-fold increase in grain number per panicle due to the restoration of the fertility of the pedicellate spikelets, which invariably abort in natural sorghum accessions. Here, we report the identification of another gene, MSD3, which is also involved in the regulation of grain numbers in sorghum. Four bulked F2 populations from crosses between BTx623 and each of the independent msd mutants p6, p14, p21, and p24 were sequenced to 20x coverage of the whole genome on a HiSeq 2000 system. Bioinformatic analyses of the sequence data showed that one gene, Sorbi_3001G407600, harbored homozygous mutations in all four populations. This gene encodes a plastidial omega-3 fatty acid desaturase that catalyzes the conversion of linoleic acid (18:2) to linolenic acid (18:3), a substrate for jasmonic acid (JA) biosynthesis. The msd3 mutants had reduced levels of linolenic acid in both leaves and developing panicles that in turn decreased the levels of JA. Furthermore, the msd3 panicle phenotype was reversed by treatment with methyl-JA (MeJA). Our characterization of MSD1, MSD2, and now MSD3 demonstrates that JA-regulated processes are critical to the msd phenotype. The identification of the MSD3 gene reveals a new target that could be manipulated to increase grain number per panicle in sorghum, and potentially other cereal crops, through the genomic editing of MSD3 functional orthologs.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation

organism description > plant
CSHL Authors:
Communities: CSHL labs > Ware lab
Depositing User: Matthew Dunn
Date: 28 October 2019
Date Deposited: 08 Nov 2019 16:39
Last Modified: 08 Nov 2019 16:39
Related URLs:
URI: http://repository.cshl.edu/id/eprint/38673

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