Genome assembly of a tropical maize inbred line provides insights into structural variation and crop improvement

Yang, N., Liu, J., Gao, Q., Gui, S., Chen, L., Yang, L., Huang, J., Deng, T., Luo, J., He, L., Wang, Y., Xu, P., Peng, Y., Shi, Z., Lan, L., Ma, Z., Yang, X., Zhang, Q., Bai, M., Li, S., Li, W., Liu, L., Jackson, D., Yan, J. (June 2019) Genome assembly of a tropical maize inbred line provides insights into structural variation and crop improvement. Nat Genet, 51 (6). pp. 1052-1059. ISSN 1061-4036

URL: https://www.ncbi.nlm.nih.gov/pubmed/31152161
DOI: 10.1038/s41588-019-0427-6

Abstract

Maize is one of the most important crops globally, and it shows remarkable genetic diversity. Knowledge of this diversity could help in crop improvement; however, gold-standard genomes have been elucidated only for modern temperate varieties. Here, we present a high-quality reference genome (contig N50 of 15.78 megabases) of the maize small-kernel inbred line, which is derived from a tropical landrace. Using haplotype maps derived from B73, Mo17 and SK, we identified 80,614 polymorphic structural variants across 521 diverse lines. Approximately 22% of these variants could not be detected by traditional single-nucleotide-polymorphism-based approaches, and some of them could affect gene expression and trait performance. To illustrate the utility of the diverse SK line, we used it to perform map-based cloning of a major effect quantitative trait locus controlling kernel weight-a key trait selected during maize improvement. The underlying candidate gene ZmBARELY ANY MERISTEM1d provides a target for increasing crop yields.

Item Type: Paper
Subjects: organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > single nucleotide polymorphism > haplotype
bioinformatics > genomics and proteomics > Mapping and Rendering > Quantitative trait locus mapping
CSHL Authors:
Communities: CSHL labs > Jackson lab
Depositing User: Matthew Dunn
Date: June 2019
Date Deposited: 06 Jun 2019 19:39
Last Modified: 06 Jun 2019 19:39
Related URLs:
URI: http://repository.cshl.edu/id/eprint/38066

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