Oral mucosal organoids as a potential platform for personalized cancer therapy

Driehuis, E., Kolders, S., Spelier, S., Lohmussaar, K., Willems, S. M., Devriese, L. A., de Bree, R., de Ruiter, E. J., Korving, J., Begthel, H., Van Es, J. H., Geurts, V., He, G. W., van Jaarsveld, R. H., Oka, R., Muraro, M. J., Vivie, J., Zandvliet, Mmjm, Hendrickx, A. P. A., Iakobachvili, N., Sridevi, P., Kranenburg, O., van Boxtel, R., Kops, Gjpl, Tuveson, D. A., Peters, P. J., van Oudenaarden, A., Clevers, H. (May 2019) Oral mucosal organoids as a potential platform for personalized cancer therapy. Cancer Discov, 9 (7). pp. 852-871. ISSN 2159-8274

URL: https://www.ncbi.nlm.nih.gov/pubmed/31053628
DOI: 10.1158/2159-8290.cd-18-1522

Abstract

Previous studies have described that tumor organoids can capture the diversity of defined human carcinoma types. Here, we describe conditions for long-term culture of human mucosal organoids. Using this protocol, a panel of 31 head and neck squamous cell carcinoma (HNSCC)-derived organoid lines was established. This panel recapitulates genetic and molecular characteristics previously described for HNSCC. Organoids retain their tumorigenic potential upon xenotransplantation. We observe differential responses to a panel of drugs including cisplatin, carboplatin, cetuximab and radiotherapy in vitro. Drug screens reveals selective sensitivity to targeted drugs that are not normally used in the treatment of HNSCC patients. These observations may inspire a personalized approach to the management of HNSCC and expand the repertoire of HNSCC drugs.

Item Type: Paper
Subjects: Investigative techniques and equipment > cell culture > cancer organoids
diseases & disorders > cancer > drugs and therapies
therapies > personalised therapy
CSHL Authors:
Communities: CSHL labs > Tuveson lab
Depositing User: Matthew Dunn
Date: 3 May 2019
Date Deposited: 22 May 2019 15:05
Last Modified: 12 Aug 2019 15:36
Related URLs:
URI: http://repository.cshl.edu/id/eprint/37826

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