Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies

Shih, M. M., Davis, F. P., Henry, G. L., Dubnau, J. (January 2019) Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the Drosophila Mushroom Bodies. G3 (Bethesda), 9 (1). pp. 81-94. ISSN 2160-1836

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URL: https://www.ncbi.nlm.nih.gov/pubmed/30397017
DOI: 10.1534/g3.118.200726

Abstract

The insect mushroom body (MB) is a conserved brain structure that plays key roles in a diverse array of behaviors. The Drosophila melanogaster MB is the primary invertebrate model of neural circuits related to memory formation and storage, and its development, morphology, wiring, and function has been extensively studied. MBs consist of intrinsic Kenyon Cells that are divided into three major neuron classes (gamma, alpha'/beta' and alpha/beta) and 7 cell subtypes (gammad, gammam, alpha'/beta'ap, alpha'/beta'm, alpha/betap, alpha/betas and alpha/betac) based on their birth order, morphology, and connectivity. These subtypes play distinct roles in memory processing, however the underlying transcriptional differences are unknown. Here, we used RNA sequencing (RNA-seq) to profile the nuclear transcriptomes of each MB neuronal cell subtypes. We identified 350 MB class- or subtype-specific genes, including the widely used alpha/beta class marker Fas2 and the alpha'/beta' class marker trio Immunostaining corroborates the RNA-seq measurements at the protein level for several cases. Importantly, our data provide a full accounting of the neurotransmitter receptors, transporters, neurotransmitter biosynthetic enzymes, neuropeptides, and neuropeptide receptors expressed within each of these cell types. This high-quality, cell type-level transcriptome catalog for the Drosophila MB provides a valuable resource for the fly neuroscience community.

Item Type: Paper
Subjects: organism description > animal > insect > Drosophila
organs, tissues, organelles, cell types and functions > tissues types and functions > mushroom body
Investigative techniques and equipment > assays > RNA-seq
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transcriptomes
CSHL Authors:
Communities: CSHL labs > Dubnau lab
Depositing User: Matthew Dunn
Date: 9 January 2019
Date Deposited: 28 Jan 2019 15:14
Last Modified: 28 Jan 2019 15:14
PMCID: PMC6325895
Related URLs:
URI: http://repository.cshl.edu/id/eprint/37666

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