Conservation of notochord gene expression across chordates: insights from the Leprecan gene family

Capellini, T. D., Dunn, M. P., Passamaneck, Y. J., Selleri, L., Di Gregorio, A. (November 2008) Conservation of notochord gene expression across chordates: insights from the Leprecan gene family. Genesis, 46 (11). pp. 683-96. ISSN 1526-954x

URL: https://www.ncbi.nlm.nih.gov/pubmed/18798549
DOI: 10.1002/dvg.20406

Abstract

The notochord is a defining character of the chordates, and the T-box transcription factor Brachyury has been shown to be required for notochord development in all chordates examined. In the ascidian Ciona intestinalis, at least 44 notochord genes have been identified as bona fide transcriptional targets of Brachyury. We examined the embryonic expression of a subset of murine orthologs of Ciona Brachyury target genes in the notochord to assess its conservation throughout chordate evolution. We focused on analyzing the Leprecan gene family, which in mouse is composed of three genes, as opposed to the single-copy Ciona gene. We found that all three mouse Leprecan genes are expressed in the notochord. Additionally, while Leprecan expression in C. intestinalis is confined to the notochord, expression of its mouse orthologs includes dorsal root ganglia, limb buds, branchial arches, and developing kidneys. These results have interesting implications for the evolution and development of chordates.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cis-regulatory elements
evolution
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL Library and Archives
Depositing User: Matthew Dunn
Date: November 2008
Date Deposited: 09 Jan 2019 21:19
Last Modified: 09 Jan 2019 21:30
PMCID: PMC3065379
Related URLs:
URI: http://repository.cshl.edu/id/eprint/37656

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