Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis

Maniatis, T., Jeffrey, A., van deSande, H. (August 1975) Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry, 14 (17). pp. 3787-94.

URL: http://www.ncbi.nlm.nih.gov/PubMed/1174504

Abstract

We describe the use of polyacrylamide gel electrophoresis to estimate chain lengths of double- and single-stranded DNA molecules in the size range 20-1000 base pairs (or nucleotides). Double-stranded DNA molecules of known length produced either by organic synthesis or by restriction endonuclease digestion of viral DNAs were used as standards.

Item Type: Paper
Subjects: Investigative techniques and equipment > assays > gel electrophoresis
Communities: CSHL labs
Depositing User: Elizabeth Pessala
Date: 26 August 1975
Date Deposited: 15 Jan 2019 15:48
Last Modified: 15 Jan 2019 15:48
URI: http://repository.cshl.edu/id/eprint/37626

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