Determination of Number of Superhelical Turns in Simian Virus 40 DNA by Gel-Electrophoresis

Keller, W. (1975) Determination of Number of Superhelical Turns in Simian Virus 40 DNA by Gel-Electrophoresis. Proceedings of the National Academy of Sciences of the United States of America, 72 (12). pp. 4876-4880.

URL: https://www.ncbi.nlm.nih.gov/pubmed/174079

Abstract

Closed-circular, superhelical DNA from simian virus 40 (SV40 DNA I) was treated with an excess of DNA-relaxing enzyme in the presence of increasing amounts of ethidium bromide (EtdBr). After removal of the ethidium, each sample consisted of a group of close-circular DNA molecules differing in their number of superhelical turns (tau) around a mean value of tau in a Gaussian-like distribution.

Item Type: Paper
Subjects: Investigative techniques and equipment > assays > gel electrophoresis
Communities: Cold Spring Harbor Laboratory of Quantitative Biology
Depositing User: Elizabeth Pessala
Date: 1975
Date Deposited: 25 Jan 2019 16:33
Last Modified: 25 Jan 2019 16:33
PMCID: PMC388835
URI: http://repository.cshl.edu/id/eprint/37612

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