Transfection of Escherichia coli by Mu DNA

Kahmann, R., Kamp, D., Zipser, D. (1976) Transfection of Escherichia coli by Mu DNA. Mol Gen Genet, 149 (3). pp. 323-8.

URL: http://www.ncbi.nlm.nih.gov/PubMed/799251

Abstract

Infectivity of Mu DNA was demonstrated in Ca+ +-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10(-7) per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.

Item Type: Paper
Subjects: organism description > bacteria > escherichia coli
CSHL Authors:
Communities: Cold Spring Harbor Laboratory of Quantitative Biology
Depositing User: Elizabeth Pessala
Date: 1976
Date Deposited: 22 Feb 2019 15:41
Last Modified: 22 Feb 2019 15:41
PMCID: PMC256058
URI: http://repository.cshl.edu/id/eprint/37568

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