Transcription of simian virus 40. 3. Mapping of "early" and "late" species of RNA

Sambrook, J., Sugden, B., Keller, W., Sharp, P. A. (December 1973) Transcription of simian virus 40. 3. Mapping of "early" and "late" species of RNA. Proc Natl Acad Sci U S A, 70 (12). pp. 3711-5.

URL: https://www.ncbi.nlm.nih.gov/pubmed/4129925

Abstract

To determine the orientation of transcription of the E and L strands of DNA from simian virus 40 (SV40), we used linear DNA prepared by cleavage of superhelical viral DNA by endonuclease R.R(1) from Escherichia coli as a primer.template for DNA polymerase. The resulting molecules, which were labeled only at the 3' end of each DNA strand, were then cleaved with Hemophilus parainfluenzae endonuclease Hpa I

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > virus
CSHL Authors:
Communities: Cold Spring Harbor Laboratory of Quantitative Biology
Depositing User: Elizabeth Pessala
Date: December 1973
Date Deposited: 11 Dec 2018 20:06
Last Modified: 11 Dec 2018 20:06
PMCID: PMC427312
URI: http://repository.cshl.edu/id/eprint/37402

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