Fragments Produced by Cleavage of Lambda Deoxyribonucleic Acid with Hemophilus-Parainfluenzae Restriction Enzyme Hpa-Ii

Allet, B. (1973) Fragments Produced by Cleavage of Lambda Deoxyribonucleic Acid with Hemophilus-Parainfluenzae Restriction Enzyme Hpa-Ii. Biochemistry, 12 (20). pp. 3972-3977.

URL: https://pubs.acs.org/doi/abs/10.1021/bi00744a029
DOI: 10.1021/bi00744a029

Abstract

One of the restricting enzymes extracted from Hemophilus parainfluenzae, HpaII, is shown to cleave lambda DNA at more than 50 sites. The resulting DNA fragments have been prepared from a variety of deletion or deletion-substitution mutants of lambda, and analyzed by polyacrylamide gel electrophoresis. The major DNA segments (larger than 0.3 Mdalton) produced by digestion of lambda DNA have been mapped and many of the cleavage sites in the immunity region, in the b2 region, and to the right of the immunity region have been identified.

Item Type: Paper
Subjects: Investigative techniques and equipment > assays > gel electrophoresis
Communities: Cold Spring Harbor Laboratory of Quantitative Biology
Depositing User: Elizabeth Pessala
Date: 1973
Date Deposited: 29 Mar 2019 16:45
Last Modified: 29 Mar 2019 16:45
URI: http://repository.cshl.edu/id/eprint/37363

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