Allet, B. (1973) Fragments Produced by Cleavage of Lambda Deoxyribonucleic Acid with Hemophilus-Parainfluenzae Restriction Enzyme Hpa-Ii. Biochemistry, 12 (20). pp. 3972-3977.
Abstract
One of the restricting enzymes extracted from Hemophilus parainfluenzae, HpaII, is shown to cleave lambda DNA at more than 50 sites. The resulting DNA fragments have been prepared from a variety of deletion or deletion-substitution mutants of lambda, and analyzed by polyacrylamide gel electrophoresis. The major DNA segments (larger than 0.3 Mdalton) produced by digestion of lambda DNA have been mapped and many of the cleavage sites in the immunity region, in the b2 region, and to the right of the immunity region have been identified.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment > assays > gel electrophoresis |
| Communities: | Cold Spring Harbor Laboratory of Quantitative Biology |
| Depositing User: | Elizabeth Pessala |
| Date: | 1973 |
| Date Deposited: | 29 Mar 2019 16:45 |
| Last Modified: | 29 Mar 2019 16:45 |
| URI: | https://repository.cshl.edu/id/eprint/37363 |
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