Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor Specimens

Baker, L. A., Tiriac, H., Tuveson, D. A. (2019) Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor Specimens. Methods Mol Biol, 1882. pp. 97-115. ISSN 1064-3745

URL: https://www.ncbi.nlm.nih.gov/pubmed/30378047
DOI: 10.1007/978-1-4939-8879-2_9

Abstract

The recent development of human organoids as patient-specific models of pancreatic ductal adenocarcinoma (PDA) has helped set the stage for a new era of personalized medicine. Organoids can be generated from a resected PDA tumor in as little as 2-4 weeks, and are amenable to therapeutic screening as well as genetic and biochemical perturbation. Moreover, because these models promote the propagation of the neoplastic PDA cells at the expense of the stromal cells, transcriptome and genome-wide sequencing of organoids offers an unprecedented view of the genetic and expression changes occurring in the neoplastic cells of individual tumors. Here, we describe methods to generate PDA organoid cultures from resected human tumor specimens. We also describe how to propagate, cryopreserve, and thaw human PDA organoid cultures.

Item Type: Paper
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > cell culture > cancer organoids
diseases & disorders > cancer > cancer types > pancreatic cancer
CSHL Authors:
Communities: CSHL labs > Tuveson lab
Depositing User: Matthew Dunn
Date: 2019
Date Deposited: 27 Nov 2018 14:56
Last Modified: 27 Nov 2018 14:56
Related URLs:
URI: http://repository.cshl.edu/id/eprint/37343

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