Amino-Acid-Catalyzed Direct Aldol Bioconjugation

Howard, T. S., Cohen, R. D., Nwajiobi, O., Muneeswaran, Z. P., Sim, Y. E., Lahankar, N. N., Yeh, J. T., Raj, M. (August 2018) Amino-Acid-Catalyzed Direct Aldol Bioconjugation. Org Lett. ISSN 1523-7052

URL: https://www.ncbi.nlm.nih.gov/pubmed/30125118
DOI: 10.1021/acs.orglett.8b02265

Abstract

A site-specific bioconjugation was developed based on direct aldol coupling using amino-acid-derived organocatalysts. The functionalization exhibits fast kinetics and occurs under mild, biocompatible conditions (viz., aqueous media, moderate temperature, and neutral pH). The resulting bioconjugates were found to be stable toward abundant aldolase enzymes, as well as acidic and basic pH. The methodology was demonstrated through conjugation of a variety of small molecules, dyes, and peptides to proteins, including a single-domain antibody, which was then used for cellular imaging.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > antibodies
CSHL Authors:
Communities: CSHL Cancer Center Shared Resources > Antibody and Phage Display Service
Depositing User: Matthew Dunn
Date: 20 August 2018
Date Deposited: 21 Aug 2018 19:27
Last Modified: 21 Aug 2018 19:27
Related URLs:
URI: http://repository.cshl.edu/id/eprint/37131

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving