Predicting Gene Structure Changes Resulting from Genetic Variants via Exon Definition Features

Majoros, W. H., Holt, C., Campbell, M. S., Ware, D., Yandell, M., Reddy, T. E. (2018) Predicting Gene Structure Changes Resulting from Genetic Variants via Exon Definition Features. Bioinformatics. ISSN 1367-4803

URL: https://www.ncbi.nlm.nih.gov/pubmed/29701825
DOI: 10.1093/bioinformatics/bty324

Abstract

Motivation: Genetic variation that disrupts gene function by altering gene splicing between individuals can substantially influence traits and disease. In those cases, accurately predicting the effects of genetic variation on splicing can be highly valuable for investigating the mechanisms underlying those traits and diseases. While methods have been developed to generate high quality computational predictions of gene structures in reference genomes, the same methods perform poorly when used to predict the potentially deleterious effects of genetic changes that alter gene splicing between individuals. Underlying that discrepancy in predictive ability are the common assumptions by reference gene finding algorithms that genes are conserved, well-formed, and produce functional proteins. Results: We describe a probabilistic approach for predicting recent changes to gene structure that may or may not conserve function. The model is applicable to both coding and noncoding genes, and can be trained on existing gene annotations without requiring curated examples of aberrant splicing. We apply this model to the problem of predicting altered splicing patterns in the genomes of individual humans, and we demonstrate that performing gene-structure prediction without relying on conserved coding features is feasible. The model predicts an unexpected abundance of variants that create de novo splice sites, an observation supported by both simulations and empirical data from RNA-seq experiments. While these de novo splice variants are commonly misinterpreted by other tools as coding or noncoding variants of little or no effect, we find that in some cases they can have large effects on splicing activity and protein products, and we propose that they may commonly act as cryptic factors in disease. Availability: The software is available from geneprediction.org/SGRF. Contact: bmajoros@duke.edu. Supplementary information: Supplementary information is available at Bioinformatics online.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs > Ware lab
Depositing User: Matt Covey
Date Deposited: 22 May 2018 16:38
Last Modified: 22 May 2018 16:38
Related URLs:
URI: http://repository.cshl.edu/id/eprint/36563

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