Transposon-derived small RNAs triggered by miR845 mediate genome dosage response in Arabidopsis

Borges, F., Parent, J. S., van Ex, F., Wolff, P., Martinez, G., Kohler, C., Martienssen, R. A. (February 2018) Transposon-derived small RNAs triggered by miR845 mediate genome dosage response in Arabidopsis. Nat Genet, 50 (2). pp. 186-192. ISSN 1061-4036

URL: https://www.ncbi.nlm.nih.gov/pubmed/29335544
DOI: 10.1038/s41588-017-0032-5

Abstract

Chromosome dosage has substantial effects on reproductive isolation and speciation in both plants and animals, but the underlying mechanisms are largely obscure (1) . Transposable elements in animals can regulate hybridity through maternal small RNA (2) , whereas small RNAs in plants have been postulated to regulate dosage response via neighboring imprinted genes(3,4). Here we show that a highly conserved microRNA in plants, miR845, targets the tRNA(Met) primer-binding site (PBS) of long terminal repeat (LTR) retrotransposons in Arabidopsis pollen, and triggers the accumulation of 21-22-nucleotide (nt) small RNAs in a dose-dependent fashion via RNA polymerase IV. We show that these epigenetically activated small interfering RNAs (easiRNAs) mediate hybridization barriers between diploid seed parents and tetraploid pollen parents (the 'triploid block'), and that natural variation for miR845 may account for 'endosperm balance' allowing the formation of triploid seeds. Targeting of the PBS with small RNA is a common mechanism for transposon control in mammals and plants, and provides a uniquely sensitive means to monitor chromosome dosage and imprinting in the developing seed.

Item Type: Paper
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: February 2018
Date Deposited: 25 Jan 2018 20:58
Last Modified: 07 Mar 2018 16:43
PMCID: PMC5805582
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35985

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving