The role of ribonucleoproteins in the production of mitotic abnormalities

Kaufmann, B. P., Das, N. K. (January 1955) The role of ribonucleoproteins in the production of mitotic abnormalities. Chromosoma, 7 (1). pp. 19-38. ISSN 0009-5915 (Print)0009-5915 (Linking)

URL: https://www.ncbi.nlm.nih.gov/pubmed/13250679
DOI: 10.1007/BF00329717

Abstract

Immersion of growing roots of onion and lily in aerated solutions of ribonuclease affected their pattern of growth and altered the structure and mitotic distribution of the chromosomes. Action of the enzyme on meristematic cells caused enlargement of nucleoli, excessive contraction, stickiness, adhesion, and clumping of chromosomes, and production of aneuploid and polyploid chromosome complexes, tripolar and multipolar spindles, binucleate and multinucleate cells. Very few cases of chromosome fragmentation were observed. Accumulation of abnormalities accompanied the passage of ribonuclease across the root as determined by alterations in stainability of the cells with pyronin and fast green. There was no visible modification of stainability of the chromosomes with methyl green or the Feulgen reagent. These results, when compared with those produced by control solutions, indicate that ribonuclease enters the living cell and degrades ribonucleoproteins essential for maintenance of structural and functional integrity. The implications of these results, with respect to the production of aberrations by other agents, are discussed.

Item Type: Paper
Uncontrolled Keywords: *Chromosomes *Nucleoproteins *Ribonucleoproteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear ribonucleoprotein
CSHL Authors:
Communities: The Carnegie Institution Department of Genetics
Depositing User: Matt Covey
Date: January 1955
Date Deposited: 23 Jan 2018 15:27
Last Modified: 23 Jan 2018 15:27
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35956

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