The corneal epithelium as a source of mammalian somatic mitosis

Gay, Helen, Kaufmann, Berwind Petersen (1950) The corneal epithelium as a source of mammalian somatic mitosis. Stain Technology, 25 (4). pp. 209-216. ISSN 0038-9153

URL: https://www.ncbi.nlm.nih.gov/pubmed/14782056
DOI: 10.3109/10520295009110992

Abstract

Dividing cells that occur abundantly in the lowermost layers of the corneal epithelium of various laboratory mammals provide a reliable and readily procurable source of mitotic figures for studies of chromosome number and form. Preparations may be secured quickly by fixing and staining entire eyes in an orcein-alcohol-acetic-acid mixture, or by fixing in acetic-alcohol and then staining with orcein or with leucobasic f uchsin as used in the Feulgen technic. For cytological examination of the epithelial cells and for determination of mitotic rates, the cornea is dissected from the eye and mounted as a flat preparation. Squashes of the epithelial cells are useful if mitotic counts are not required.

Item Type: Paper
Uncontrolled Keywords: Animals *Cell Division *Chromosomes *Coloring Agents *Epithelium *Epithelium, Corneal *Mitosis *Staining and Labeling *Stains and staining
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
CSHL Authors:
Communities: The Carnegie Institution Department of Genetics
Depositing User: Matt Covey
Date Deposited: 23 Jan 2018 15:53
Last Modified: 23 Jan 2018 15:53
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35936

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