Successful creation of pancreatic cancer organoids by means of EUS-guided fine-needle biopsy for personalized cancer treatment

Tiriac, Herve, Bucobo, Juan Carlos, Tzimas, Demetrios, Grewel, Suman, Lacomb, Joseph F., Rowehl, Leahana M., Nagula, Satish, Wu, Maoxin, Kim, Joseph, Sasson, Aaron, Vignesh, Shivakumar, Martello, Laura, Munoz-Sagastibelza, Maria, Somma, Jonathan, Tuveson, David A., Li, Ellen, Buscaglia, Jonathan M. (2018) Successful creation of pancreatic cancer organoids by means of EUS-guided fine-needle biopsy for personalized cancer treatment. Gastrointestinal Endoscopy, 87 (6). pp. 1474-1480. ISSN 0016-5107

URL: https://www.ncbi.nlm.nih.gov/pubmed/29325707
DOI: 10.1016/j.gie.2017.12.032

Abstract

AbstractBackground and Aims Pancreatic cancer organoids are tumor models of individualized human pancreatic ductal adenocarcinoma (PDA), created from surgical specimens and used for personalized treatment strategies. Unfortunately the vast majority of patients with PDA are not operative candidates. Creation of human PDA organoids at the time of initial tumor diagnosis is therefore critical. Our aim was to assess the feasibility of creating human PDA organoids by EUS fine-needle biopsy (EUS-FNB) in patients with PDA. Methods Prospective clinical trial in patients referred to evaluate a pancreatic mass (ClincalTrials.gov: NCT01698190). EUS-FNA was performed for initial on-site diagnosis. Two additional needle passes were performed with a 22-gauge FNB needle for organoid creation. Primary outcome was successful isolation of organoids within 2 weeks of EUS-FNB (P0), confirmed by organoid morphology and positive genotyping. Results Thirty-seven patients with 38 PDA tumors were enrolled. Successful isolation of organoids (P0) was achieved in 33 of 38 tumors, or 87%. Establishment of PDA organoid lines for ≥5 passages of growth (P5) was reached in 25 of 38 tumors, or 66%. In the single patient with successful P5 FNB-derived and P5 surgically derived organoids, there was identical matching of specimens. There were no serious adverse events. Two patients developed bleeding at the EUS-FNB puncture site requiring hemostasis clips. Conclusions Pancreatic cancer organoids can be successfully and rapidly created by means of EUS-FNB using a 22-gauge needle at the time of initial diagnosis. Successful organoid generation is essential for precision medicine in patients with pancreatic cancer, in whom the majority are not surgically resectable.

Item Type: Paper
Uncontrolled Keywords: EUS Organoids EUS-FNB Pancreatic Cancer Pancreatic Adenocarcinoma
Subjects: Investigative techniques and equipment > cell culture > cancer organoids
organism description > model organism
diseases & disorders > cancer > cancer types > pancreatic cancer
CSHL Authors:
Communities: CSHL Cancer Center Program > Signal Transduction
CSHL labs > Tuveson lab
Depositing User: Matt Covey
Date Deposited: 12 Jan 2018 19:46
Last Modified: 14 Jun 2018 21:07
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35811

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