Epigenetic control of pheromone MAPK signaling determines sexual fecundity in Candida albicans

Scaduto, C. M., Kabrawala, S., Thomson, G. J., Scheving, W., Ly, A., Anderson, M. Z., Whiteway, M., Bennett, R. J. (December 2017) Epigenetic control of pheromone MAPK signaling determines sexual fecundity in Candida albicans. Proc Natl Acad Sci U S A, 114 (52). pp. 13780-13785. ISSN 0027-8424

[img]
Preview
PDF (Paper)
Scaduto 2017.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (957Kb) | Preview
URL: https://www.ncbi.nlm.nih.gov/pubmed/29255038
DOI: 10.1073/pnas.1711141115

Abstract

Several pathogenic Candida species are capable of heritable and reversible switching between two epigenetic states, "white" and "opaque." In Candida albicans, white cells are essentially sterile, whereas opaque cells are mating-proficient. Here, we interrogate the mechanism by which the white-opaque switch regulates sexual fecundity and identify four genes in the pheromone MAPK pathway that are expressed at significantly higher levels in opaque cells than in white cells. These genes encode the beta subunit of the G-protein complex (STE4), the pheromone MAPK scaffold (CST5), and the two terminal MAP kinases (CEK1/CEK2). To define the contribution of each factor to mating, C. albicans white cells were reverse-engineered to express elevated, opaque-like levels of these factors, either singly or in combination. We show that white cells co-overexpressing STE4, CST5, and CEK2 undergo mating four orders of magnitude more efficiently than control white cells and at a frequency approaching that of opaque cells. Moreover, engineered white cells recapitulate the transcriptional and morphological responses of opaque cells to pheromone. These results therefore reveal multiple bottlenecks in pheromone MAPK signaling in white cells and that alleviation of these bottlenecks enables efficient mating by these "sterile" cell types. Taken together, our findings establish that differential expression of several MAPK factors underlies the epigenetic control of mating in C. albicans We also discuss how fitness advantages could have driven the evolution of a toggle switch to regulate sexual reproduction in pathogenic Candida species.

Item Type: Paper
Uncontrolled Keywords: mating phenotypic switching sexual reproduction signaling bottlenecks transcriptional regulation
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > epigenetics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > epigenetics

organism description > yeast
Communities: CSHL labs > Sheltzer lab
Depositing User: Matt Covey
Date: 26 December 2017
Date Deposited: 12 Jan 2018 17:10
Last Modified: 25 Oct 2018 16:14
PMCID: PMC5748179
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35808

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving