Advances in Serodiagnostic Testing for Lyme Disease Are at Hand

Branda, J. A., Body, B. A., Boyle, J., Branson, B. M., Dattwyler, R. J., Fikrig, E., Gerald, N. J., Gomes-Solecki, M., Kintrup, M., Ledizet, M., Levin, A. E., Lewinski, M., Liotta, L. A., Marques, A., Mead, P. S., Mongodin, E. F., Pillai, S., Rao, P., Robinson, W. H., Roth, K. M., Schriefer, M. E., Slezak, T., Snyder, J., Steere, A. C., Witkowski, J., Wong, S. J., Schutzer, S. E. (March 2018) Advances in Serodiagnostic Testing for Lyme Disease Are at Hand. Clin Infect Dis, 66 (7). pp. 1133-1139. ISSN 1058-4838

URL: https://www.ncbi.nlm.nih.gov/pubmed/29228208
DOI: 10.1093/cid/cix943

Abstract

The cause of Lyme disease, Borrelia burgdorferi, was discovered in 1983. A 2-tiered testing protocol was established for serodiagnosis in 1994, involving an enzyme immunoassay (EIA) or indirect fluorescence antibody, followed (if reactive) by immunoglobulin M and immunoglobulin G Western immunoblots. These assays were prepared from whole-cell cultured B. burgdorferi, lacking key in vivo expressed antigens and expressing antigens that can bind non-Borrelia antibodies. Additional drawbacks, particular to the Western immunoblot component, include low sensitivity in early infection, technical complexity, and subjective interpretation when scored by visual examination. Nevertheless, 2-tiered testing with immunoblotting remains the benchmark for evaluation of new methods or approaches. Next-generation serologic assays, prepared with recombinant proteins or synthetic peptides, and alternative testing protocols, can now overcome or circumvent many of these past drawbacks. This article describes next-generation serodiagnostic testing for Lyme disease, focusing on methods that are currently available or near-at-hand.

Item Type: Paper
Uncontrolled Keywords: Borrelia burgdorferi Lyme disease diagnosis serology tests
Subjects: diseases & disorders
diseases & disorders > Bacterial Infections > Lyme disease
Investigative techniques and equipment > serology
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: March 2018
Date Deposited: 14 Dec 2017 18:00
Last Modified: 06 Apr 2018 14:41
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35721

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