Boundary Formation through a Direct Threshold-Based Readout of Mobile Small RNA Gradients

Skopelitis, Damianos S., Benkovics, Anna H., Husbands, Aman Y., Timmermans, Marja C. P. (November 2017) Boundary Formation through a Direct Threshold-Based Readout of Mobile Small RNA Gradients. Developmental Cell, 43 (3). pp. 265-273. ISSN 1534-5807

URL: https://www.ncbi.nlm.nih.gov/pubmed/29107557
DOI: 10.1016/j.devcel.2017.10.003

Abstract

Summary Small RNAs have emerged as a new class of mobile signals. Here, we investigate their mechanism of action and show that mobile small RNAs generate sharply defined domains of target gene expression through an intrinsic and direct threshold-based readout of their mobility gradients. This readout is highly sensitive to small RNA levels at the source, allowing plasticity in the positioning of a target gene expression boundary. Besides patterning their immediate targets, the readouts of opposing small RNA gradients enable specification of robust, uniformly positioned developmental boundaries. These patterning properties of small RNAs are reminiscent of those of animal morphogens. However, their mode of action and the intrinsic nature of their gradients distinguish mobile small RNAs from classical morphogens and present a unique direct mechanism through which to relay positional information. Mobile small RNAs and their targets thus emerge as highly portable, evolutionarily tractable regulatory modules through which to create pattern.

Item Type: Paper
Uncontrolled Keywords: microRNA morphogen intercellular signaling pattern formation robustness adaxial-abaxial polarity leaf development
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > leaf polarity
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

organism description > plant behavior > plant orientation > plant polarity
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:
Communities: CSHL labs > Timmermans lab
CSHL labs > Vakoc lab
Depositing User: Matt Covey
Date: 6 November 2017
Date Deposited: 30 Oct 2017 19:18
Last Modified: 15 Nov 2017 20:26
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35650

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