Antisense Oligonucleotide-mediated Knockdown in Mammary Tumor Organoids

Diermeier, S. D., Spector, D. L. (August 2017) Antisense Oligonucleotide-mediated Knockdown in Mammary Tumor Organoids. Bio Protoc, 7 (16). ISSN 2331-8325 (Print)2331-8325

URL: https://www.ncbi.nlm.nih.gov/pubmed/28932764
DOI: 10.21769/BioProtoc.2511

Abstract

Primary mammary tumor organoids grown in 3D are an excellent system to study tumor biology. They resemble the organization and physiology of native epithelia more closely than cancer cell lines grown in 2D, and additionally model interactions with the ECM (Boj et al., 2015; Clevers, 2016; Shamir and Ewald, 2014). Mammary tumor organoids are therefore a promising model system to identify and characterize novel drivers of breast cancer that would be unlikely to be identified using 2D cell lines. Antisense oligonucleotides can be used to efficiently and specifically knockdown target genes in the cell (Bennett et al., 2017). They can be taken up freely by organoids without the need for a transfection agent, making them a convenient tool for routine lab studies and screens.

Item Type: Paper
Uncontrolled Keywords: 3D cell culture Antisense knockdown Antisense oligonucleotides Breast cancer Mammary tumor Organoids
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > antisense
diseases & disorders > cancer > cancer types > breast cancer
Investigative techniques and equipment > cell culture
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > oligonucleotide
CSHL Authors:
Communities: CSHL labs > Spector lab
CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
Depositing User: Matt Covey
Date: 20 August 2017
Date Deposited: 25 Sep 2017 15:37
Last Modified: 12 Jun 2018 19:03
PMCID: PMC5602589
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35464

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