Two regions of the adenovirus early region 1A proteins are required for transformation

Whyte, P., Ruley, H. E., Harlow, E. (January 1988) Two regions of the adenovirus early region 1A proteins are required for transformation. J Virol, 62 (1). pp. 257-65. ISSN 0022-538x

URL: http://www.ncbi.nlm.nih.gov/pubmed/2960834

Abstract

Regions of the adenovirus type 5 early region 1A (E1A) proteins that are required for transformation were defined by using a series of deletion mutants. Deletion mutations collectively spanning the entire protein-coding region of E1A were constructed and assayed for their ability to cooperate with an activated ras oncogene to induce transformation in primary baby rat kidney cells. Two regions of E1A (amino acids 1 to 85 and 121 to 127) were found to be essential for transformation. Deletion of all or part of the region from amino acids 121 to 127 resulted in a total loss of transforming ability. An adjacent stretch of amino acids (residues 128 to 139), largely consisting of acidic residues, was found to be dispensable for transformation but appeared to influence the efficiency of transformation. Amino acids 1 to 85 made up a second region of the E1A protein that was essential for transformation. Deletion of all or part of this region resulted in a loss of the transforming activity. Even a mutation resulting in a single amino acid change at position 2 of the polypeptide chain was sufficient to eliminate transformation. Deletion of amino acids 86 to 120 or 128 to 289 did not eliminate transformation, although some mutations in these regions had lowered efficiencies of transformation. Foci induced by transformation-competent mutants could be expanded into cell lines that retained their transformed morphology and constitutively expressed the mutant E1A proteins.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins Animals Cell Line *Cell Transformation, Viral DNA Mutational Analysis *Genes, ras Oncogene Proteins, Viral/*physiology Rats Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Structure-Activity Relationship
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > E1A protein
organism description > virus > adenovirus
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell transformation
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: January 1988
Date Deposited: 05 Sep 2017 15:50
Last Modified: 05 Sep 2017 15:50
PMCID: PMC250526
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35228

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