Activation of the U2 snRNA promoter by the octamer motif defines a new class of RNA polymerase II enhancer elements

Tanaka, M., Grossniklaus, U., Herr, W., Hernandez, N. (December 1988) Activation of the U2 snRNA promoter by the octamer motif defines a new class of RNA polymerase II enhancer elements. Genes Dev, 2 (12B). pp. 1764-78. ISSN 0890-9369

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URL: http://www.ncbi.nlm.nih.gov/pubmed/2853685
DOI: 10.1101/gad.2.12b.1764

Abstract

The recent discovery that the activation domains of transcriptional activators (e.g., GAL4) from a number of species are interchangeable has led to the concept of a general mechanism for activation of RNA polymerase II genes. We have examined the different activities of the SV40 octamer motif ATGCAAAG in B cells and in HeLa cells in the context of either the beta-globin promoter, a TATA-box-containing mRNA promoter, or the U2 snRNA promoter, which contains a snRNA-specific proximal element. In the context of the beta-globin promoter, the octamer motif is a B-cell-specific enhancer element, whereas it is a ubiquitous enhancer element for the U2 snRNA promoter. The U2 promoter is unique in that it is not activated by enhancer elements that activate the beta-globin promoter, and a hybrid U2 promoter containing the upstream activating sequence UASG is not stimulated by a yeast GAL4 trans-activator. Together, these observations suggest that in the context of the U2 promoter, the octamer motif defines a new class of RNA polymerase II enhancer elements, which bind transcription factors that trans-activate gene expression by a different mechanism than the general mechanism mentioned above. These results are discussed in light of the possibility that the ubiquitous octamer binding protein Oct-1 and the B-cell-specific octamer binding protein Oct-2 are involved in the activation of the U2 and beta-globin promoters, respectively.

Item Type: Paper
Uncontrolled Keywords: B-Lymphocytes *Enhancer Elements (Genetics) Fungal Proteins/genetics Globins/genetics Hela Cells Humans Models, Genetic Plasmids *Promoter Regions (Genetics) RNA Polymerase II/*genetics RNA, Small Nuclear/*genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. *Saccharomyces cerevisiae Proteins Simian virus 40/genetics Simplexvirus/genetics Transcription Factors/genetics Transfection Viral Proteins/genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Gail Sherman
Date: December 1988
Date Deposited: 14 Sep 2017 20:20
Last Modified: 03 Nov 2017 21:07
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35221

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