The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain

Sturm, R. A., Das, G., Herr, W. (December 1988) The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain. Genes Dev, 2 (12A). pp. 1582-99. ISSN 0890-9369

[img]
Preview
PDF (Paper)
Herr Genes Dev 1988b.pdf - Published Version

Download (5Mb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/2905684
DOI: 10.1101/gad.2.12a.1582

Abstract

The octamer motif ATGCAAAT is recognized indistinguishably by two mammalian transcription factors: one that is expressed ubiquitously and referred to here as Oct-1, and another, Oct-2, that is expressed in lymphoid cells. We report the cDNA cloning of the human oct-1 gene, which encodes Oct-1, by screening lambda gt11 recombinant phage in situ for octamer motif-specific DNA binding. One lambda gt11 recombinant expressed a beta-galactosidase-octamer-binding fusion protein with a DNA-binding specificity indistinguishable from human HeLa cell Oct-1 protein. As expected for a ubiquitously expressed protein, Oct-1 mRNA is expressed in all five human and two mouse cell lines tested. Polyclonal rabbit antiserum raised against the beta-galactosidase fusion protein shows that the DNA-binding domains of Oct-1 and Oct-2 proteins are related antigenically. Deletion analysis of the 743-amino-acid-long oct-1 open reading frame shows that the DNA-binding activity lies within a central highly charged domain of 160 amino acids. Comparison of the Oct-1 and Oct-2 sequences reveals that this domain is nearly identical between the two proteins. Highly similar domains are also present in the pituitary-specific transcription factor Pit-1 and the Caenorhabditis elegans unc-86 cell lineage gene product (see Herr et al. 1988). Within this shared POU (Pit-1, Oct-1 and Oct-2, unc-86) domain (pronounced 'pow') lie two subdomains: a POU-related homeo box and a POU-specific box. The Oct-1 protein is unique among the POU-related proteins and other homeo box proteins because it is expressed ubiquitously.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Bacteriophage lambda/genetics Base Sequence Binding Sites Cell Line Cloning, Molecular Comparative Study DNA-Binding Proteins/*genetics Genes, Homeobox *Genes, Structural Humans Immunoblotting Lymphoid Tissue/cytology Mice Molecular Sequence Data Nuclear Proteins/*genetics Recombinant Fusion Proteins/genetics/immunology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Restriction Mapping Transcription Factors/genetics beta-Galactosidase/genetics
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
organism description > animal
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Gail Sherman
Date: December 1988
Date Deposited: 14 Sep 2017 20:08
Last Modified: 03 Nov 2017 21:06
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35219

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving