Growth factor induction by the adenovirus type 5 E1A 12S protein is required for immortalization of primary epithelial cells

Quinlan, M. P., Whyte, P., Grodzicker, T. (August 1988) Growth factor induction by the adenovirus type 5 E1A 12S protein is required for immortalization of primary epithelial cells. Molecular & Cellular Biology, 8 (8). pp. 3191-203. ISSN 0270-7306

URL: http://www.ncbi.nlm.nih.gov/pubmed/2974923

Abstract

The 12S protein encoded by the adenovirus E1A region induces cellular DNA synthesis in and proliferation and immortalization of primary rat epithelial cells in the presence or absence of serum. It also induces the production of a growth factor(s) that stimulates epithelial cell proliferation. We have undertaken a mutational analysis of the 12S gene to determine the sequences required for these functions. We found that a region near the C-terminus of the 12S protein was required for growth factor induction. No activities have been defined previously for this region. Furthermore, we show that growth factor production was necessary for epithelial cells to survive past their normal life span in culture and to become immortalized. The ability to induce growth factor production required prior expression of E1A activities encoded by the N-terminus of the 12S protein, including activation of quiescent cells into the cell cycle, and an unknown activity that required expression of the first 13 amino acids of the gene. In addition, examination of the subcellular localization of mutant 12S polypeptides suggested new regions that affect the nuclear localization of E1A proteins.

Item Type: Paper
Subjects: organism description > virus > adenovirus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > epithelial cells
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: August 1988
Date Deposited: 18 Sep 2017 16:35
Last Modified: 18 Sep 2017 16:35
PMCID: PMC363551
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35199

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