Multiple phosphorylated forms of the product of the fission yeast cell division cycle gene cdc2+

Potashkin, J. A., Beach, D. H. (September 1988) Multiple phosphorylated forms of the product of the fission yeast cell division cycle gene cdc2+. Curr Genet, 14 (3). pp. 235-40. ISSN 0172-8083

URL: http://www.ncbi.nlm.nih.gov/pubmed/3058333
DOI: 10.1007/BF00376743

Abstract

The 34 kilodalton protein product (p34) of the cdc2+ cell cycle control gene of Schizosaccharomyces pombe was expressed in bacteria. Monoclonal antibodies raised against this protein are capable of immunoprecipitating p34cdc2 from yeast lysates. Immunoprecipitates of [35S]methionine- and [32P]orthophosphate-labeled p34cdc2 were analyzed by two-dimensional gel electrophoresis. The cdc2+ gene product is homogeneous in size but resolves into seven species of differing charge. At least four of these species are phosphorylated. Phosphoamino acid analysis reveals that phosphorylation occurs mainly on threonine residues. The pattern of p34 phosphorylation is unaltered at the nonpermissive temperature in strains carrying temperature sensitive alleles of weel-50 and ran1-114 or in a strain overproducing the ran1+ gene product.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2
Investigative techniques and equipment > assays > gel electrophoresis
organism description > yeast > Saccharomyces > Schizosaccharomyces pombe
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: September 1988
Date Deposited: 26 Sep 2017 19:07
Last Modified: 26 Sep 2017 19:07
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35197

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