Effects of mutations in stem and loop regions on the structure and function of adenovirus VA RNAI

Mellits, K. H., Mathews, M. B. (September 1988) Effects of mutations in stem and loop regions on the structure and function of adenovirus VA RNAI. Embo J, 7 (9). pp. 2849-59. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/3181142
DOI: doi/10.1002/j.1460-2075.1988.tb03141.x/abstract

Abstract

Adenovirus virus-associated (VA) RNAI is required for efficient protein synthesis at late times of adenoviral infection, and in some other situations where double-stranded RNA (dsRNA) is present. It prevents inhibition of protein synthesis by a dsRNA-activated protein kinase and the secondary structure of VA RNAI is though to be important for its activity. To test this idea and to define structures and sequences responsible for VA RNAI activity, we constructed several mutant VA RNA genes and tested them in a transient expression assay. Activity is unaffected by deletions within a small region near the center of the gene, nt 72-85, but it is greatly diminished by deletion or substitution of sequences on the 3' side of this region. The structures of wild-type and mutant RNAs were examined by nuclease-sensitivity analysis. We propose a model for wild-type VA RNAI which differs from that predicted to be the most stable structure. Surprisingly disruption of the longest duplex region in the molecule is tolerated, provided that adjacent structural elements are not rearranged. However, perturbations of elements located in the center of the structure correlate well with loss of function.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: 1 September 1988
Date Deposited: 26 Sep 2017 19:27
Last Modified: 26 Sep 2017 19:27
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35180

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving