Ultrastructure and Electron Immunocytochemistry of Insulin-Producing B-Cell Tumors from Transgenic Mice - Comparison with Counterpart Human-Tumors

Holm, R., Varndell, I. M., Power, R. F., Bishop, A. E., Madsen, O. D., Alpert, S., Hanahan, D., Polak, J. M. (September 1988) Ultrastructure and Electron Immunocytochemistry of Insulin-Producing B-Cell Tumors from Transgenic Mice - Comparison with Counterpart Human-Tumors. Ultrastructural Pathology, 12 (5). pp. 547-559. ISSN 0191-3123

URL: http://www.ncbi.nlm.nih.gov/pubmed/2848349
DOI: 10.3109/01913128809032239

Abstract

This paper reports on an ultrastructural and electron-microscopic immunocytochemical study of pancreatic B cells from normal mice, pancreatic B cells and derivative tumors from transgenic mice, and tissue from human pancreatic B-cell tumors. In normal and neoplastic B cells from both species, typical immature and mature beta-granules (with spherical cores of variable density) were observed, whereas typical beta-granules with a crystalloid core were only present in human B cells (normal and tumor). A small number of atypical granules were found in distinct neoplastic cells which contained no typical beta-granules. The atypical granules were smaller (100-200 nm diameter) than typical beta-granules (250-450 nm diameter) seen in other cells. Immunoreactivity for proinsulin was localized only to immature granules, whereas insulin and C-peptide immunoreactivities were demonstrated in atypical, immature, and mature granules. In transgenic mouse and human B-cell tumors, insulin immunoreactivity was consistently weaker than the immunostaining for C-peptide. An intragranular, topographic segregation of immunoreactive C-peptide was observed in a population of transgenic tumor cells. Our results showed similarities in antigenic distribution and only slight differences in morphology between human and mouse B cells. Therefore, the transgenic mouse system may prove to be an effective model for studying mammalian B-cell tumorigenesis

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells

organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types

organism description > animal > mammal > primates > hominids > human
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: September 1988
Date Deposited: 29 Sep 2017 15:16
Last Modified: 29 Sep 2017 15:16
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35165

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