A Genetic-Physical Map of the Rhodobacter-Capsulatus Carotenoid Biosynthesis Gene Cluster

Giuliano, G., Pollock, D., Stapp, H., Scolnik, P. A. (July 1988) A Genetic-Physical Map of the Rhodobacter-Capsulatus Carotenoid Biosynthesis Gene Cluster. Molecular & General Genetics, 213 (1). pp. 78-83. ISSN 0026-8925

URL: http://link.springer.com/article/10.1007%2FBF00333...
DOI: 10.1007/BF00333401

Abstract

We used the interposon mutagenesis technique to map a cluster of Rhodobacter capsulatus genes that are responsible for the biosynthesis of carotenoids, membrane pigments that protect living organisms from photooxidations. Fifteen interposons define 7 genes (crtA, crtI, crtB, crtC, crtD, crtE and crtF) in an approximately 8 kb of the chromosome. This cluster is flanked by genes affecting bacteriochlorophyll biosynthesis. Based on the analysis of the phenotypes of the insertional mutants and the results of complementation analyses, we present a genetic and physical map of this region. Our data indicate that, with the possible exception of crtB, all genes analyzed are organized as single transcriptional units. We used nuclease S1 protection analysis to study the influence of oxygen on the regulation of expression of 4 genes (crtA, crtI, crtC and crtE). Under anaerobic conditions, mRNA levels for crtA, crtc and crtE are elevated and a new crtE transcript is detected.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > annotation > map annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: July 1988
Date Deposited: 12 Oct 2017 18:46
Last Modified: 12 Oct 2017 18:46
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35157

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