The c-fos serum response element responds to protein kinase C-dependent and -independent signals but not to cyclic AMP

Gilman, M. Z. (April 1988) The c-fos serum response element responds to protein kinase C-dependent and -independent signals but not to cyclic AMP. Genes Dev, 2 (4). pp. 394-402. ISSN 0890-9369

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URL: http://www.ncbi.nlm.nih.gov/pubmed/2453393
DOI: 10.1101/gad.2.4.394

Abstract

Transcription of the c-fos proto-oncogene is rapidly induced by serum growth factors. A short c-fos DNA element, the serum response element (SRE), is required for this response to serum. However, serum activates a series of distinct intracellular signaling pathways, and it is not known to which of these pathways the SRE responds. To address this question, mutations have been introduced into the SRE of an otherwise intact c-fos promoter/enhancer. These mutations strongly reduce the binding of a nuclear factor to this site. Plasmids carrying either a wild-type or mutant c-fos SRE were transfected into fibroblasts and tested for their response to whole serum, purified recombinant c-sis protein, the protein kinase C activator phorbol myristate acetate, and activators of the cyclic AMP (cAMP) second messenger system. Assays were carried out under normal conditions and after chronic phorbol ester-treatment to deplete phorbol ester activatable protein kinase C activity from transfected cells. The results show that the SRE is necessary and sufficient for response to both protein kinase C-dependent and -independent intracellular signaling pathways but not for response to the cAMP pathway.

Item Type: Paper
Uncontrolled Keywords: 1-Methyl-3-isobutylxanthine/pharmacology Animals Base Sequence Blood/metabolism Cyclic AMP/*metabolism Fibroblasts/metabolism Forskolin/pharmacology *Gene Expression Regulation Growth Substances/metabolism Mice Mice, Inbred BALB C Mutation Oligonucleotides/genetics Promoter Regions (Genetics) Protein Kinase C/*metabolism *Proto-Oncogenes Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Tetradecanoylphorbol Acetate/pharmacology Transfection
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > c-fos
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor > Cyclic AMP
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: April 1988
Date Deposited: 12 Oct 2017 17:56
Last Modified: 03 Nov 2017 21:10
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35155

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