Synthesis of p34, the mammalian homolog of the yeast cdc2+/CDC28 protein kinase, is stimulated during adenovirus-induced proliferation of primary baby rat kidney cells

Draetta, G., Beach, D., Moran, E. (June 1988) Synthesis of p34, the mammalian homolog of the yeast cdc2+/CDC28 protein kinase, is stimulated during adenovirus-induced proliferation of primary baby rat kidney cells. Oncogene, 2 (6). pp. 553-7. ISSN 0950-9232

URL: http://www.ncbi.nlm.nih.gov/pubmed/2838787

Abstract

The homolog of the cdc2+/CDC28+ encoded protein kinases has previously been identified in HeLa cells by immunological methods. Here we have studied the distribution and synthesis of this 34 kd protein in rat tissues and cell lines. p34 was found in a variety of organs, including some such as brain that are not highly active in cell division. Kidney has very low levels of p34. However, proliferative activation of baby rat kidney cells with adenovirus caused rapid induction of p34 synthesis. Induction was dependent on the E1A gene of the virus but not E1B, and was not prevented by inhibition of cellular DNA synthesis with hydroxyurea. Increased synthesis of p34 is due, at least in part, to an increase in abundance of translatable p34 mRNA. These data are consistent with the possibility that p34 plays a role in cell division in higher vertebrates.

Item Type: Paper
Uncontrolled Keywords: Adenoviridae/physiology Adenoviridae Infections/*physiopathology Animals *Cell Cycle Cell Line Cells, Cultured Immunologic Techniques Protein Kinases/*physiology RNA, Messenger/genetics Rats Research Support, U.S. Gov't, P.H.S. Tissue Distribution
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Gail Sherman
Date: June 1988
Date Deposited: 06 Oct 2017 15:27
Last Modified: 06 Oct 2017 15:27
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35135

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