Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement

Draetta, G., Beach, D. (1988) Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement. Cell, 54 (1). pp. 17-26. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/3289755
DOI: 10.1016/0092-8674(88)90175-4

Abstract

HeLa cell p34, homolog of the yeast cdc2+/CDC28 protein kinase, has been investigated. p34 was phosphorylated at two or more sites and existed in a complex with p13, the previously identified homolog of the suc1+ gene product of S. pombe. A fraction of the most highly phosphorylated form of p34 was also associated with p62, a newly identified protein that became phosphorylated in vitro. The phosphorylation state of p34, its association with p62, and the protein kinase activity of the complex were each subject to cell cycle regulation. In newly born cells early in G1, p34 was unphosphorylated, not associated with p62, and inactive as a protein kinase. Each of these conditions was reversed in G2 and the p34/p62 complex was maximally active as a protein kinase, with respect to both endogenous and exogenous substrates, during mitotic metaphase. p34 may act to regulate the G2/M transition in HeLa cells.

Item Type: Paper
Uncontrolled Keywords: Antineoplastic Agents/pharmacology Benzimidazoles/pharmacology CDC2 Protein Kinase Caseins/metabolism *Cell Cycle Centrifugation Chromatography, Gel Electrophoresis, Polyacrylamide Gel Enzyme Activation Flow Cytometry Hela Cells Humans Immunoassay Metaphase/drug effects Microtubules/drug effects *Mitosis Nocodazole Phosphoproteins/*metabolism Phosphorylation Protein Kinases/*metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
organism description > animal > mammal > primates > hominids > human
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Gail Sherman
Date Deposited: 17 Oct 2017 17:10
Last Modified: 17 Oct 2017 17:10
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35134

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