Stimulation of exocytotic degranulation by microinjection of the ras oncogene protein into rat mast cells

Bar-Sagi, D., Gomperts, B. D. (October 1988) Stimulation of exocytotic degranulation by microinjection of the ras oncogene protein into rat mast cells. Oncogene, 3 (4). pp. 463-9. ISSN 0950-9232

URL: http://www.ncbi.nlm.nih.gov/pubmed/3078954

Abstract

To investigate the possible role of ras proteins in the secretory process, we have microinjected the proto oncogenic and oncogenic forms of the human H-ras protein into rat peritoneal mast cells. Mast cells are secretory cells which, upon appropriate stimulus, liberate histamine and other mediators of the acute inflammatory reaction by exocytotic degranulation. We report here that microinjection of the ras oncogene protein into mast cells induces exocytotic degranulation. In contrast, microinjection of similar amounts of the proto-oncogenic protein has little apparent effect on mast cells. Degranulation induced by injection of the ras oncogene protein occurs in the absence of an external stimulus and requires the presence of external calcium. The ultrastructural features of exocytotic degranulation in mast cells injected with the ras oncogene protein are similar to those seen when mast cells are activated by soluble ligands. Our results suggest that ras proteins may be involved, possibly as regulatory elements, in cellular functions that control exocytosis.

Item Type: Paper
Uncontrolled Keywords: Animals Cytoplasmic Granules/ultrastructure *Exocytosis *Genes, ras Humans Kinetics Mast Cells/*physiology/ultrastructure Microinjections Microscopy, Electron Oncogene Protein p21(ras)/administration & dosage/*metabolism Rats Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Time Factors
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Ras
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: October 1988
Date Deposited: 10 Nov 2017 16:44
Last Modified: 10 Nov 2017 16:44
URI: http://repository.cshl.edu/id/eprint/35117

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