The preparation of prolactin

Bates, Robert Wesley, Riddle, Oscar (November 1935) The preparation of prolactin. The Journal of Pharmacology and Experimental Therapeutics , 55 (3). pp. 365-371.

URL: http://jpet.aspetjournals.org/content/55/3/365

Abstract

A procedure for extraction and proximate purification of prolactin is fully described. The tissue is first extracted with 60 to 70 per cent aqueous ethanol at pH 9 to 10, and this followed by complete precipitation of the active principles at a higher ethanol concentration and at pH 6.0. The prolactin is next separated from the bulk of follicle-stimulating and thyreotropic hormones by taking advantage of the insolubility of prolactin between pH 3 to 4 in the presence of sulphates. The hormone is further purified through use of its solubility in aqueous ethanol. About 70 per cent of the prolactin present in the original tissue is thus obtained in one fraction uncontaminated by F. S. H. and thyreotropic hormone. At pH 8.0 prolactin can be heated to boiling for one hour or to 60°C. for five hours without great loss of potency.

Item Type: Paper
Subjects: Investigative techniques and equipment
CSHL Authors:
Communities: Station for Experimental Evolution at Cold Spring Harbor
Depositing User: Elizabeth Pessala
Date: November 1935
Date Deposited: 04 Jul 2017 00:30
Last Modified: 04 Jul 2017 00:30
URI: http://repository.cshl.edu/id/eprint/34928

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