Cellular targets for transformation by the adenovirus E1A proteins

Whyte, P., Williamson, N. M., Harlow, E. (January 1989) Cellular targets for transformation by the adenovirus E1A proteins. Cell, 56 (1). pp. 67-75. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/2521301
DOI: 10.1016/0092-8674(89)90984-7

Abstract

Three cellular proteins, including species of 300,000 daltons and 107,000 daltons as well as p105-RB, the product of the retinoblastoma susceptibility gene, stably interact with the adenovirus E1A proteins. To help determine the functional basis of these interactions, the regions of E1A that participate in these interactions were mapped using a series of deletion mutants. The 300,000 dalton and the 107,000 dalton proteins interacted with sequences within amino acids 1 to 76 and 121 to 127, respectively. Interaction with the third cellular protein, p105-RB, required the presence of sequences from two noncontiguous regions of the E1A polypeptide chain, amino acids 30 to 60 and 121 to 127. The regions of E1A that are required for these interactions coincided precisely with the regions of E1A that are required for its transforming function. These results suggest that the interactions with these cellular proteins are fundamental to the transforming activity of E1A.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins *Cell Transformation, Viral DNA Mutational Analysis Hela Cells Humans In Vitro Molecular Weight Nuclear Proteins/physiology Oncogene Proteins, Viral/*physiology Precipitin Tests Protein Binding Research Support, U.S. Gov't, P.H.S. Retinoblastoma/*genetics Structure-Activity Relationship
Subjects: organism description > virus > adenovirus
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell transformation
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: 13 January 1989
Date Deposited: 23 Jun 2017 15:37
Last Modified: 23 Jun 2017 15:37
Related URLs:
URI: http://repository.cshl.edu/id/eprint/34916

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